February 1, 2000AJP Heart and Circulatory Physiology
Expression of the β (slow)-isoform of MHC in the adult mouse heart causes dominant-negative functional effects
Why the study?
Does genetic expression of the beta-isoform of MHC decrease cardiac contractility in adult mouse hearts?
Population
Adult mouse heart model (transgenic mouse lines)
Comparison
Genetic alteration to express a Myc-tagged… vs Normal adult mouse ventricles expressing only…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Small beta-MHC shifts warrant no clinical action; leaves open their role in human contractile dysfunction.
Structured PICO
Does genetic expression of the beta-isoform of MHC decrease cardiac contractility in adult mouse hearts?
P
Population
Adult mouse heart model (transgenic mouse lines)
I
Intervention
Genetic alteration to express a Myc-tagged beta-MHC molecule (the slower motor) in adult ventricular tissue (12% of myosin as transgene)
C
Comparator
Normal adult mouse ventricles expressing only alpha-MHC
O
Outcome
Changes in cardiac chamber mass and contractility (myofibrillar Ca(2+)-activated ATPase activity and systolic function)surrogate
Even small shifts in the myosin isoform composition of the myocardium (such as 12% beta-MHC expression) can result in physiologically significant decreases in cardiac contractility without inducing hypertrophy.
Cite This Study
Tardiff et al. (2000) studied this question.