What question did this study set out to answer?

To identify the role of carbohydrate ligands in mediating the adherence of Plasmodium falciparum-infected erythrocytes to host cell surfaces.

July 1, 1995The Journal of Experimental MedicineOpen Access

Chondroitin sulfate A is a cell surface receptor for Plasmodium falciparum-infected erythrocytes.

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Authors

SRStephen J. RogersonPeter Doherty Institute SCSansanee C. ChaiyarojMahidol University KNKen NgThe University of Melbourne

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Implication

Experimental study demonstrates specific binding of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A, suggesting a key molecular pathway for parasite cytoadherence in malaria.

Key Points

To identify the role of carbohydrate ligands in mediating the adherence of Plasmodium falciparum-infected erythrocytes to host cell surfaces.
Measured binding of infected erythrocytes to wild-type Chinese hamster ovary (CHO) cells, C32 melanoma cells, and glycosylation-mutant CHO cell lines lacking chondroitin sulfate A.
Conducted inhibition assays using soluble glycosaminoglycans and evaluated adherence following chondroitinase digestion of cell surfaces.
Tested specific adherence of laboratory-selected parasite lines and clinical patient isolates to immobilized chondroitin sulfate A.
Chondroitinase treatment of wild-type CHO cells reduced infected erythrocyte adherence by up to 90%, and mutant cells lacking chondroitin sulfate A supported little or no binding.
Soluble chondroitin sulfate A inhibited parasite adherence to CHO cells by 99.2 ± 0.2% at 10 mg/ml and 72.5 ± 3.8% at 1 mg/ml, whereas other glycosaminoglycans showed no inhibitory effect.
Parasite lines selected for cell binding as well as most clinical patient isolates adhered specifically to immobilized chondroitin sulfate A.

Cite This Study

Rogerson et al. (1995) studied this question.

synapsesocial.com/papers/69d8f7b46715230d10bedc49 https://doi.org/10.1084/jem.182.1.15

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Implication

Experimental study demonstrates specific binding of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A, suggesting a key molecular pathway for parasite cytoadherence in malaria.

Chondroitin sulfate A is a cell surface receptor for Plasmodium falciparum-infected erythrocytes.

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Implication

Key Points

Cite This Study

Chondroitin sulfate A is a cell surface receptor for Plasmodium falciparum-infected erythrocytes.

Discussion

Member takes

Implication

Key Points

Cite This Study

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