What question did this study set out to answer?

To identify the precise cellular and ultrastructural site of the blood-brain barrier to protein tracers in the mammalian cerebral cortex.

July 1, 1967The Journal of Cell BiologyOpen Access

Fine Structural Localization of a Blood-Brain Barrier to Exogenous Peroxidase

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To identify the precise cellular and ultrastructural site of the blood-brain barrier to protein tracers in the mammalian cerebral cortex.
Administered horseradish peroxidase intravenously to mice across varying doses.
Fixed brain tissue by immersion or vascular perfusion between 10 and 60 minutes post-injection.
Mapped peroxidase tracer localization across cerebral cortical microvessels using electron microscopy.
Exogenous peroxidase localized exclusively within the lumina of cortical vessels and within rare endothelial micropinocytotic vesicles, with none escaping beyond the vascular endothelium.
Endothelial tight junctions prevented the intercellular diffusion of peroxidase into the brain parenchyma, establishing the vascular endothelium as the physical barrier.

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Implication

Ultrastructural study reveals that vascular endothelial tight junctions block exogenous peroxidase passage in mouse cerebral cortex, demonstrating an endothelial blood-brain barrier.

Cite This Study

Reese et al. (1967) studied this question.

synapsesocial.com/papers/69d9073d183921ebcaae46d7 https://doi.org/10.1083/jcb.34.1.207

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Implication

Ultrastructural study reveals that vascular endothelial tight junctions block exogenous peroxidase passage in mouse cerebral cortex, demonstrating an endothelial blood-brain barrier.

Fine Structural Localization of a Blood-Brain Barrier to Exogenous Peroxidase

Key Points

Discussion

Implication

Cite This Study

Fine Structural Localization of a Blood-Brain Barrier to Exogenous Peroxidase

Key Points

Discussion

Implication

Cite This Study