What question did this study set out to answer?

To determine the distinct effects of thrombin and platelet-derived growth factor (PDGF) on cellular growth, signaling pathways, and immediate-early gene expression in pulmonary arterial smooth muscle cells.

March 1, 1994Journal of Biological ChemistryOpen Access

Immediate-early gene expression in response to hypertrophic and proliferative stimuli in pulmonary arterial smooth muscle cells.

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Authors

ARAbraham RothmanUniversity of Nevada, Las Vegas BWBranden S. WolnerUniversity of Massachusetts Chan Medical School DBDonald ButtonRoche (Switzerland)

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Implication

In vitro study reveals distinct gene responses to thrombin and PDGF in pulmonary arterial smooth muscle cells, suggesting divergent signaling in vascular remodeling.

Key Points

To determine the distinct effects of thrombin and platelet-derived growth factor (PDGF) on cellular growth, signaling pathways, and immediate-early gene expression in pulmonary arterial smooth muscle cells.
Treated rat pulmonary arterial smooth muscle cells (PAC1) with thrombin (1 unit/ml) or PDGF (2.5 ng/ml) over time periods up to 5 days.
Quantified protein synthesis, cellular protein content, and cell proliferation, and evaluated immediate-early gene mRNA expression via Northern blotting under various signaling inhibition conditions.
Thrombin induced cellular hypertrophy with a 102 ± 12% increase in protein synthesis and a 49 ± 11% increase in protein content per cell without altering cell counts, whereas PDGF stimulated cell proliferation (cell doubling in 5 days) without altering cellular protein content.
Both agonists induced egr-1, c-fos, c-jun, junB, and fra-1 mRNAs within 15 minutes (peaking at 2.9- to 9.3-fold increases over control at 30–60 min), but only thrombin induced fosB mRNA (7.7 ± 4.0-fold increase, n=12, p < 0.0005).
Immediate-early gene induction generally required extracellular Ca2+, Na+/H+ exchange, and protein kinase C, while thapsigargin-sensitive intracellular Ca2+ stores were specifically required for thrombin signaling.

Cite This Study

Rothman et al. (1994) studied this question.

synapsesocial.com/papers/69d921107fca1f84ab6843ea https://doi.org/10.1016/s0021-9258(17)37385-4

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Implication

In vitro study reveals distinct gene responses to thrombin and PDGF in pulmonary arterial smooth muscle cells, suggesting divergent signaling in vascular remodeling.

Immediate-early gene expression in response to hypertrophic and proliferative stimuli in pulmonary arterial smooth muscle cells.

Discussion

Member takes

Implication

Key Points

Cite This Study

Immediate-early gene expression in response to hypertrophic and proliferative stimuli in pulmonary arterial smooth muscle cells.

Discussion

Member takes

Implication

Key Points

Cite This Study

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