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Quantify the diversity, abundance, and mobilization potential of antibiotic resistance genes across manure processing and land disposal stages in commercial swine facilities.

February 11, 2013Proceedings of the National Academy of SciencesOpen Access

Diverse and abundant antibiotic resistance genes in Chinese swine farms

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Authors

YZYong‐Guan ZhuSouth China Agricultural University TJTimothy A. JohnsonPurdue University West Lafayette JSJian-Qiang SuInstitute of Urban Environment

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Implication

Environmental surveillance reveals massive enrichment of diverse antibiotic resistance genes in commercial swine farm waste, indicating high risks of environmental dissemination.

Key Points

Quantify the diversity, abundance, and mobilization potential of antibiotic resistance genes across manure processing and land disposal stages in commercial swine facilities.
Sampled waste at three manure processing stages through land application across three large-scale commercial swine farms (10,000 animals per year) in China alongside antibiotic-free controls.
Profiled resistance determinants and mobile genetic elements using high-capacity quantitative PCR arrays targeting 149 unique antibiotic resistance genes.
Quantified concentrations of veterinary antibiotics and heavy metal feed additives to assess selection pressure across manure samples.
High-capacity qPCR detected 149 unique resistance genes, with the top 63 genes enriched 192-fold (median) up to 28,000-fold (maximum) relative to antibiotic-free control samples.
Top six transposase alleles showed enrichment up to 90,000-fold (median 189-fold) in manure and correlated strongly with resistance gene abundance (r² = 0.96).
Direct correlations linked elevated concentrations of antibiotics and heavy metal feed supplements with resistance gene abundance, indicating coselection mechanisms.

Cite This Study

Zhu et al. (2013) studied this question.

synapsesocial.com/papers/69d922cfe0d31bb747835dae https://doi.org/10.1073/pnas.1222743110

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Implication

Environmental surveillance reveals massive enrichment of diverse antibiotic resistance genes in commercial swine farm waste, indicating high risks of environmental dissemination.

Diverse and abundant antibiotic resistance genes in Chinese swine farms

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Cite This Study

Diverse and abundant antibiotic resistance genes in Chinese swine farms

Discussion

Member takes

Implication

Key Points

Cite This Study

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