What question did this study set out to answer?

The aim is to reduce sequencing errors and improve the reliability of 16S rRNA-based microbial studies.

Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies

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Randomized trial evaluates methods to improve sequencing accuracy in microbial community analysis, suggesting enhanced data reliability.

The aim is to reduce sequencing errors and improve the reliability of 16S rRNA-based microbial studies.
Analyzed 2.7 million reads from 90 identical mock community samples.
Evaluated methods for identifying and correcting sequence errors.
Implemented the PyroNoise algorithm for improved sequence accuracy.
Average error rate reduced from 0.0060 to 0.0002 using the PyroNoise algorithm.
Chimeric sequences identified decreased from 8% to 1% after quality filtering.
Observed an increase in spurious operational taxonomic units with more sequencing effort.

Schloss et al. (2011) studied this question.

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