June 5, 2008Journal of Clinical MicrobiologyOpen Access

Sensitive Detection of Multiple Rotavirus Genotypes with a Single Reverse Transcription-Real-Time Quantitative PCR Assay

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Population

159 stool samples and isolates representing prevalent human rotavirus genotypes

Comparison

Reverse transcription-real-time quantitative PCR… vs Classical rotavirus detection by enzyme-linked…

Design

Other

Authors

IGIon Gutiérrez‐AguirreNational Institute of Biology ASAndrej SteyerNational Laboratory of Health, Environment and Food JBJ. BobenAgricultural institute of Slovenia

Discussion

Member takes

Implication

May aid rotavirus research detection; leaves open clinical adoption pending validation studies.

Structured PICO

Population

159 stool samples and isolates representing prevalent human rotavirus genotypes (G1P[8], G2P[4], G3P[8], G4P[8], G9P[8], and G12P[8])

Intervention

Reverse transcription (RT)-real-time quantitative PCR approach targeted to the rotaviral VP2 gene

Comparator

Classical rotavirus detection by enzyme-linked immunosorbent assay and nested RT-PCR

Outcome

Detection sensitivity and broad range detection of rotavirus strainssurrogate

A newly developed RT-qPCR assay targeting the VP2 gene offers highly sensitive and broad-range detection of multiple rotavirus genotypes, outperforming classical ELISA and nested RT-PCR methods.

Cite This Study

Gutiérrez‐Aguirre et al. (2008) studied this question.

synapsesocial.com/papers/69d92569da3af5b1d0835cf5 https://doi.org/10.1128/jcm.02428-07

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