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Synapse
November 18, 2013Cell Communication and Signaling494 citationsOpen Access

Exosomes are natural carriers of exogenous siRNA to human cells in vitro

TSTatiana ShtamRKR. A. KovalevEVE. Yu. Varfolomeeva

Key Points

  • To investigate whether human exosomes can serve as natural delivery vehicles for exogenous siRNA to knock down cancer therapeutic targets in vitro.
  • Assessed in vitro delivery of exogenous small interfering RNA (siRNA) into target human cells using exosomes.
  • Targeted the RAD51 gene to evaluate functional RNA interference (RNAi) mediated knockdown.
  • Exosomes effectively transported exogenous siRNA into target human cells in vitro.
  • Exosome-mediated siRNA delivery produced strong knockdown of the cancer-associated target gene RAD51.

Abstract

The results strongly suggest that exosomes effectively delivered the siRNA into the target cells. The therapeutic potential of exosome-mediated siRNA delivery was demonstrated in vitro by the strong knockdown of RAD51, a prospective therapeutic target for cancer cells. The results give an additional evidence of the ability to use human exosomes as vectors in cancer therapy, including RNAi-based gene therapy.

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Cite This Study

Shtam et al. (2013) studied this question.

synapsesocial.com/papers/69d94ace16f0d2beeba3c4cbhttps://doi.org/10.1186/1478-811x-11-88
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