What question did this study set out to answer?

To characterize the lipolytic substrate specificity and regulatory properties of endothelial lipase in comparison to lipoprotein lipase and hepatic lipase.

June 1, 2002SHILAP Revista de lepidopterologíaOpen Access

Characterization of the lipolytic activity of endothelial lipase

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Population

Conditioned medium from cells infected with recombinant adenoviruses encoding endothelial lipase…

Comparison

Endothelial lipase (EL) vs Lipoprotein lipase (LPL) and hepatic lipase (HL)

Design

Preclinical

Authors

MMMary McCoyUniversity of California, Merced GSGwo-Shing SunUniversity of Pennsylvania DMDawn MarchadierTranslational Therapeutics (United States)

Discussion

Member takes

Implication

EL modulation remains investigational; leaves open human translation of its HDL preference from animal data.

Key Points

To characterize the lipolytic substrate specificity and regulatory properties of endothelial lipase in comparison to lipoprotein lipase and hepatic lipase.
Assayed lipolytic and phospholipase activities using radiolabeled lipid substrates and conditioned media from cells infected with recombinant adenoviruses expressing endothelial lipase, lipoprotein lipase, or hepatic lipase.
Tested the regulatory effects of serum, apolipoprotein C-II, and 1 M NaCl on lipase activity.
Measured free fatty acid generation following incubation with isolated lipoprotein fractions (chylomicrons, VLDL, IDL, LDL, and HDL).
Endothelial lipase exhibited a triglyceride lipase to phospholipase activity ratio of 0.65, compared with ratios of 24.1 for hepatic lipase and 139.9 for lipoprotein lipase.
Both lipase activities of endothelial lipase were dose-dependently inhibited by serum and 1 M NaCl, but were unaffected by apolipoprotein C-II.
Endothelial lipase preferentially hydrolyzed HDL over other lipoprotein fractions, whereas lipoprotein lipase and hepatic lipase efficiently hydrolyzed triglyceride-rich lipoproteins (chylomicrons, VLDL, and IDL).

Structured PICO

Population

Conditioned medium from cells infected with recombinant adenoviruses encoding endothelial lipase (EL), lipoprotein lipase (LPL), and hepatic lipase (HL)

Intervention

Endothelial lipase (EL)

Comparator

Lipoprotein lipase (LPL) and hepatic lipase (HL)

Outcome

Lipolytic activity (triglyceride lipase and phospholipase activities) and ability to hydrolyze lipids in isolated lipoprotein fractionssurrogate

Endothelial lipase exhibits a unique lipolytic profile with a low triglyceride lipase to phospholipase activity ratio and preferentially hydrolyzes HDL.

Cite This Study

McCoy et al. (2002) studied this question.

synapsesocial.com/papers/69d94ce49402b8412aa3cdcf https://doi.org/10.1016/s0022-2275(20)30466-1

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Characterization of the lipolytic activity of endothelial lipase

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Characterization of the lipolytic activity of endothelial lipase

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider