What question did this study set out to answer?

To identify the structural domains and transport mechanisms responsible for targeting and retaining emerin within the inner nuclear membrane.

June 1, 1999Journal of Cell Science

Intracellular trafficking of emerin, the Emery-Dreifuss muscular dystrophy protein

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Authors

CÖCecilia ÖstlundColumbia University JEJan EllenbergCentre National de la Recherche Scientifique EHEinar HallbergStockholm University

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Implication

In vitro imaging reveals that the amino-terminal domain targets emerin to the inner nuclear membrane, suggesting a diffusion-retention model relevant to Emery-Dreifuss muscular dystrophy.

Key Points

To identify the structural domains and transport mechanisms responsible for targeting and retaining emerin within the inner nuclear membrane.
Assessed the subcellular localization of wild-type, truncated, and chimeric emerin constructs using confocal immunofluorescence microscopy.
Measured diffusion coefficients (D) of green fluorescent protein-tagged emerin in the inner nuclear membrane, endoplasmic reticulum, and plasma membrane using fluorescence photobleaching.
The nucleoplasmic amino-terminal domain of emerin proved necessary and sufficient for inner nuclear membrane retention, whereas its transmembrane segment alone showed no nuclear targeting.
Emerin exhibited lower diffusional mobility in the inner nuclear membrane (D = 0.10 ± 0.01 µm²/second) compared with the endoplasmic reticulum membrane (D = 0.32 ± 0.01 µm²/second).

Cite This Study

Östlund et al. (1999) studied this question.

synapsesocial.com/papers/69d96a011ad561c6736841d2 https://doi.org/10.1242/jcs.112.11.1709

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Implication

In vitro imaging reveals that the amino-terminal domain targets emerin to the inner nuclear membrane, suggesting a diffusion-retention model relevant to Emery-Dreifuss muscular dystrophy.

Intracellular trafficking of emerin, the Emery-Dreifuss muscular dystrophy protein

Discussion

Member takes

Implication

Key Points

Cite This Study

Intracellular trafficking of emerin, the Emery-Dreifuss muscular dystrophy protein

Discussion

Member takes

Implication

Key Points

Cite This Study

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