What question did this study set out to answer?

To develop a human embryonic stem cell-derived culture model that reproduces dorsomedial telencephalic development to generate functional hippocampal neurons.

November 17, 2015Nature CommunicationsOpen Access

Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue

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Authors

HSHideya SakaguchiRIKEN Center for Biosystems Dynamics Research TKTaisuke KadoshimaKobe Asahi Hospital MSMika SoenNagoya University

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Implication

In vitro study demonstrates generation of functional hippocampal neurons from human embryonic stem cells, highlighting a novel platform for modeling brain development and disease.

Key Points

To develop a human embryonic stem cell-derived culture model that reproduces dorsomedial telencephalic development to generate functional hippocampal neurons.
Treated human embryonic stem cells (hESCs) with titrated bone morphogenetic protein (BMP) and Wnt signaling to induce choroid plexus and self-organizing medial pallium tissues.
Maintained differentiated dorsomedial telencephalic tissues in long-term dissociation culture to assess hippocampal neuron specification and electrophysiological activity.
Titrating BMP and Wnt pathway exposure successfully enabled the self-organization of medial pallium tissue in vitro.
Long-term dissociation culture yielded Zbtb20(+)/Prox1(+) granule-like and Zbtb20(+)/KA1(+) pyramidal-like hippocampal neurons that were electrically functional and established interconnected networks.

Cite This Study

Sakaguchi et al. (2015) studied this question.

synapsesocial.com/papers/69d96e69da3af5b1d083686e https://doi.org/10.1038/ncomms9896

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Implication

In vitro study demonstrates generation of functional hippocampal neurons from human embryonic stem cells, highlighting a novel platform for modeling brain development and disease.

Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue

Discussion

Member takes

Implication

Key Points

Cite This Study

Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue

Discussion

Member takes

Implication

Key Points

Cite This Study

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