Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
December 15, 2016SHILAP Revista de lepidopterologíaOpen Access

Outer Membrane Vesicles and Soluble Factors Released by Probiotic Escherichia coli Nissle 1917 and Commensal ECOR63 Enhance Barrier Function by Regulating Expression of Tight Junction Proteins in Intestinal Epithelial Cells

View Full Paper
Ask AI
Bookmark
Share

Authors

CÁCarina Shianya Álvarez-VillagómezUniversidad Juárez Autónoma de TabascoJBJosefa Badı́aSant Joan de Déu Research FoundationMBManel BoschUniversitat de Barcelona

Discussion

Loading...

Member takes

Implication

In vitro study reveals that bacterial outer membrane vesicles and soluble factors enhance barrier integrity in intestinal epithelial cells, indicating multiple pathways for gut protection.

Key Points

  • To determine whether outer membrane vesicles and soluble factors secreted by probiotic Escherichia coli Nissle 1917 and commensal ECOR63 reinforce intestinal epithelial barrier integrity by modulating tight junction proteins.
  • Fractionated cell-free supernatants from wild-type and tcpC-mutant Escherichia coli Nissle 1917 and ECOR63 into isolated outer membrane vesicles (OMVs) and soluble secreted factors.
  • Assessed intestinal barrier integrity by measuring transepithelial resistance and tight junction protein expression (ZO-1, ZO-2, claudin-14, and claudin-2) in polarized T-84 and Caco-2 monolayers.
  • Both OMVs and soluble factors from EcN and ECOR63 promoted the upregulation of tight junction proteins ZO-1 and claudin-14, while downregulating pore-forming claudin-2.
  • The barrier-strengthening effects of OMVs occurred independently of the protein TcpC, whereas soluble TcpC contributed to ZO-1 and claudin-14 upregulation without affecting claudin-2 transcription.

Cite This Study

Álvarez-Villagómez et al. (2016) studied this question.

synapsesocial.com/papers/69d972648988aeabbe685618https://doi.org/10.3389/fmicb.2016.01981
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Epithelial crosstalk at the microbiota–mucosal interface2010 · 589 citations
  2. 2Proteomic analysis of outer membrane vesicles from the probiotic strain Escherichia coli Nissle 19172013 · 97 citations
  3. 3Cdx2 transcription factor regulates claudin‐3 and claudin‐4 expression during intestinal differentiation of gastric carcinoma2008 · 60 citations
  4. 4The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 19172015 · 30 citations