What question did this study set out to answer?

Determine whether small nucleolar RNAs systematically produce conserved small regulatory RNAs across diverse eukaryotic lineages.

May 27, 2009RNAOpen Access

Small RNAs derived from snoRNAs

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Authors

RTRyan J. TaftGenetic Alliance EGEvgeny A. GlazovThe University of Queensland TLTimo LassmannThe Kids Research Institute Australia

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Implication

Cross-species genomic analysis reveals widespread generation of small RNAs from snoRNAs across eukaryotes, highlighting ancient interplay between snoRNA processing and RNA silencing.

Key Points

Determine whether small nucleolar RNAs systematically produce conserved small regulatory RNAs across diverse eukaryotic lineages.
Profiled small RNAs derived from snoRNA loci across multiple organisms, including human, mouse, chicken, fruit fly, Arabidopsis, and fission yeast.
Assessed Argonaute binding (AGO7 in Arabidopsis, Ago1 in fission yeast) and evaluated processing dependencies using Drosophila loquacious and Dicer-2 mutants, along with mouse Dicer1 and Dgcr8 mutants.
Identified conserved sno-derived RNAs (sdRNAs) across all examined eukaryotes, with H/ACA snoRNAs generating 20–24 nucleotide fragments predominantly from their 3′ ends and C/D snoRNAs yielding bimodal 17–19 and >27 nucleotide fragments primarily from their 5′ ends.
Confirmed that sdRNAs physically associate with Argonaute proteins with specific 5′ nucleotide biases and exhibit altered biogenesis in Dicer and DGCR8 mutant models, identifying sdRNAs as an ancient class of small RNAs.

Cite This Study

Taft et al. (2009) studied this question.

synapsesocial.com/papers/69d99d34a1d151c65f684994 https://doi.org/10.1261/rna.1528909

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Implication

Cross-species genomic analysis reveals widespread generation of small RNAs from snoRNAs across eukaryotes, highlighting ancient interplay between snoRNA processing and RNA silencing.

Small RNAs derived from snoRNAs

Discussion

Member takes

Implication

Key Points

Cite This Study

Small RNAs derived from snoRNAs

Discussion

Member takes

Implication

Key Points

Cite This Study

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