The Evolution of Duplicated Genes of the Cpi-17/Phi-1 (ppp1r14) Family of Protein Phosphatase 1 Inhibitors in Teleosts
Why the study?
To better understand the mechanisms of myosin phosphatase regulation through analysis of zebrafish homologs of the Cpi-17 family.
Population
Zebrafish homologs of the Cpi-17 family
Comparison
Cpi-17 paralogs vs Phi-1 paralogs
Design
In silico, in vitro, and heterologous cellular preclinical study
Authors
Discussion
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Implication
Zebrafish PHIN domain data are hypothesis-generating; leaves open any role in human smooth muscle or cardiovascular therapeutics.
Structured PICO
P
Population
Zebrafish homologs of the Cpi-17 family (in vitro and heterologous cellular systems, early embryonic development)
I
Intervention
Deletion and chimeric analysis of Cpi-17 and Phi-1 paralogs
O
Outcome
Myosin phosphatase inhibitory activity and spatial expression patternssurrogate
The study identifies biochemical and developmental distinctions between zebrafish Cpi-17 and Phi-1 paralogs, showing that the PHIN domain encodes specificity for myosin phosphatase inhibition.
Cite This Study
Lang et al. (2020) studied this question.