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November 25, 2003BloodOpen Access

Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions

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Why the study?

What novel proteins are released by thrombin-activated human platelets, and do they localize to atherosclerotic lesions?

Population

Human platelets and human atherosclerotic lesions

Comparison

Thrombin activation (in vitro proteomics approach) vs Normal vasculature (for tissue localization)

Design

Preclinical

Authors

JCJudith A. CoppingerRoyal College of Surgeons in Ireland GCGerard CagneyUniversity College Dublin STSinéad ToomeyRoyal College of Surgeons in Ireland

Discussion

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Implication

May offer novel atherosclerosis targets; animal data leave open clinical translation pending human studies.

Key Points

To comprehensively profile the proteins secreted by activated human platelets and investigate their presence in human atherosclerotic tissue.
Applied a proteomics approach to isolate and identify proteins secreted by human platelets following activation with thrombin.
Evaluated candidates for platelet localization and compared protein presence in normal vasculature versus human atherosclerotic lesions.
Identified more than 300 proteins released by thrombin-stimulated platelets, including molecules not previously attributed to platelets.
Confirmed that novel platelet proteins, including secretogranin III, cyclophilin A, and calumenin, are released upon activation and localize specifically in human atherosclerotic lesions while absent in normal vasculature.

Structured PICO

What novel proteins are released by thrombin-activated human platelets, and do they localize to atherosclerotic lesions?

P

Population

Human platelets and human atherosclerotic lesions

I

Intervention

Thrombin activation (in vitro proteomics approach)

C

Comparator

Normal vasculature (for tissue localization)

O

Outcome

Identification of proteins comprising the platelet releasatesurrogate

The identification of novel proteins released by activated platelets that localize to atherosclerotic lesions provides potential new therapeutic targets for atherosclerosis and thrombosis.

Cite This Study

Coppinger et al. (2003) studied this question.

synapsesocial.com/papers/69da22030f32475823a3d024 https://doi.org/10.1182/blood-2003-08-2804

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