What question did this study set out to answer?

To optimize expansion and differentiation culture conditions for primary normal human bronchial epithelial cells while preserving their functional 3D phenotypic characteristics.

January 24, 2019Scientific ReportsOpen Access

Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies

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Authors

RRRachael E. RaynerThe Ohio State University PMPatrudu MakenaResearch Applications (United States)GPG. L. PrasadScientific Consulting Group

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Implication

In vitro study demonstrates preserved differentiation and ion channel function across serial passages, indicating robust protocols for respiratory modeling.

Key Points

To optimize expansion and differentiation culture conditions for primary normal human bronchial epithelial cells while preserving their functional 3D phenotypic characteristics.
Cultured and serially passaged primary normal human bronchial epithelial (NHBE) cells using PneumaCult-Ex Plus expansion medium and PneumaCult-ALI differentiation medium.
Evaluated morphological and physiological endpoints across passages, including epithelial differentiation, trans-epithelial electrical resistance (TEER), ciliary beat frequency (CBF), airway surface liquid (ASL) meniscus length, and CFTR ion channel activity.
Optimal differentiation occurred with PneumaCult-Ex Plus and PneumaCult-ALI, maintaining pseudostratified columnar epithelium with ciliated and goblet cells through passage four.
Passaged cultures up to passage four maintained TEER >400 Ohms·cm² and CFTR-mediated short-circuit currents >3 µA/cm².
Ciliary beat frequency and ASL meniscus length exhibited no changes up to passage six.

Cite This Study

Rayner et al. (2019) studied this question.

synapsesocial.com/papers/69da2593b48bb130d468450a https://doi.org/10.1038/s41598-018-36735-z

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Implication

In vitro study demonstrates preserved differentiation and ion channel function across serial passages, indicating robust protocols for respiratory modeling.

Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies

Discussion

Member takes

Implication

Key Points

Cite This Study

Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies

Discussion

Member takes

Implication

Key Points

Cite This Study

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