April 15, 2008Molecular TherapyOpen Access

Analysis of AAV Serotypes 1–9 Mediated Gene Expression and Tropism in Mice After Systemic Injection

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Population

Male mice

Comparison

Adeno-associated virus pseudotyped 1-9 with a… vs Comparison among different AAV serotypes (1-9)

Design

Preclinical

Follow-up

100 days

Authors

CZCarmela ZincarelliFondazione Salvatore Maugeri SSStephen SoltysSouthern Illinois University School of Medicine GRGiuseppe RengoHeart Failure & Transplant

Discussion

Member takes

Implication

Hypothesis-generating for AAV serotype selection in murine cardiac gene therapy; leaves open translation to larger animals or humans.

Structured PICO

Population

Male mice

Intervention

Adeno-associated virus (AAV) pseudotyped 1-9 with a cytomegalovirus (CMV)-luciferase transgene via tail vein injection

Comparator

Comparison among different AAV serotypes (1-9)

Outcome

Transgene expression levels and kinetics (measured by bioluminescence imaging) and biodistribution (protein expression and viral genome copy numbers in dissected tissues)surrogate

AAV9 and AAV7 demonstrate the highest systemic transgene expression and rapid onset kinetics following tail vein injection in mice, with AAV9 showing the best viral genome distribution.

Cite This Study

Zincarelli et al. (2008) studied this question.

synapsesocial.com/papers/69da26050f32475823a3d253 https://doi.org/10.1038/mt.2008.76

Topics

Echocardiography

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Analysis of AAV Serotypes 1–9 Mediated Gene Expression and Tropism in Mice After Systemic Injection

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Topics

Analysis of AAV Serotypes 1–9 Mediated Gene Expression and Tropism in Mice After Systemic Injection

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Topics

Also Consider