MiR-1-3p that correlates with left ventricular function of HCM can serve as a potential target and differentiate HCM from DCM

Figures

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  Fig. 1. Expression of miRNAs in HCM, DCM and Control LV. The sample number of each group is 10 and p values were obtained with ANOVA test between three groups. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant (p ≥ 0.05). HCM hypertrophic cardiomyopathy; DCM dilated cardiomyopathy

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  Fig. 2. Different expression pattern of miRNAs for HCM and DCM. a miR-214 was detected downregulated in DCM compared with Control. b miR-21 was upregulated in DCM compared with Control. c, d miR-27a and miR-1-3p were upregulated in HCM compared with DCM. The sample number of each group is 10 and p values were obtained with ANOVA test *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant (p ≥ 0.05). HCM hypertrophic cardiomyopathy; DCM dilated cardiomyopathy

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  Fig. 3. ROC curve analysis of microRNAs. a–c ROC curve analysis of miR-155, miR-10b and miR-23a showed high sensitivity and specificity in discriminating disease group (HCM, DCM) with AUC ranged from 0.925 to 0.933. d ROC curve analysis of miR-21 showed AUC was notably reaching 0.944 which could distinguish DCM from Control. e–f ROC curve analysis of miR-27a and miR-1-3p showed high sensitivity and specificity in discriminating HCM from Control. Area under the curve (AUC) > 0.7 was considered accepted. HCM hypertrophic cardiomyopathy; DCM dilated cardiomyopathy

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  Fig. 4. Validate the association between miR-1-3p and indices of left heart function in a second HCM group. a, b The expression value of miR-1-3p in 17 HCM patients inversely correlated with LVEDD and directly correlated with LVEF. The Pearson correlation coefficient (r) and the relative computed p value are displayed on the graphs

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  Fig. 5. Validation of Clcn3 as a direct target of miR-1-3p. Luciferase reporter assay in 293T cells. 3′UTR sequences form Clcn3 containing predicted miR-1-3p recognition sequences were cloned downstream of luciferase. The results demonstrated as firefly luciferase activity versus Renilla luciferase activity. The relative activity was reduced by a co-transfected miR-1-3p plasmid. Student’s t test was used to analyze the results, n = 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001