What question did this study set out to answer?

To determine the structural organization, filament polarity, and membrane-attachment mechanisms of actin filament complexes in the brush border of intestinal epithelial cells.

December 1, 1975The Journal of Cell BiologyOpen Access

Organization of an actin filament-membrane complex. Filament polarity and membrane attachment in the microvilli of intestinal epithelial cells.

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Authors

MMMark S. MoosekerHeart Failure / Cardiomyopathy LTL G TilneyUniversity of Pennsylvania

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Implication

Structural analysis reveals uniform actin filament polarity and periodic membrane cross-bridges in intestinal microvilli, indicating a coordinated framework for nonmuscle cell motility.

Key Points

To determine the structural organization, filament polarity, and membrane-attachment mechanisms of actin filament complexes in the brush border of intestinal epithelial cells.
Isolated and demembranated brush borders from intestinal epithelial cells for ultrastructural examination.
Decorated actin filaments with myosin subfragment S1 to determine structural filament polarity.
Treated isolated brush borders with high concentrations of magnesium ions to induce actin paracrystals and resolve periodic cross-bridges.
All microvillar actin filaments display identical polarity, with myosin subfragment S1 arrowheads consistently pointing away from the apical tip attachment site.
Actin filaments attach end-on to the apical membrane tip and link laterally to the plasma membrane via periodic 33-nm cross-bridges along their length.
Myosin-like filaments were identified within the terminal web region underlying the microvillar actin bundles.

Cite This Study

Mooseker et al. (1975) studied this question.

synapsesocial.com/papers/69da29320f778bd2e468461e https://doi.org/10.1083/jcb.67.3.725

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Implication

Structural analysis reveals uniform actin filament polarity and periodic membrane cross-bridges in intestinal microvilli, indicating a coordinated framework for nonmuscle cell motility.

Organization of an actin filament-membrane complex. Filament polarity and membrane attachment in the microvilli of intestinal epithelial cells.

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Key Points

Cite This Study

Organization of an actin filament-membrane complex. Filament polarity and membrane attachment in the microvilli of intestinal epithelial cells.

Discussion

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Implication

Key Points

Cite This Study

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