What question did this study set out to answer?

To determine whether Aurora-B mediates cleavage furrow-specific phosphorylation of vimentin and to assess its functional role in intermediate filament segregation during cytokinesis.

February 28, 2003Journal of Biological ChemistryOpen Access

Aurora-B Regulates the Cleavage Furrow-specific Vimentin Phosphorylation in the Cytokinetic Process

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Authors

HGHidemasa GotoMie University YYYoshihiro YasuiOsaka Prefectural Institute of Public Health AKAie KawajiriAichi Cancer Center

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Implication

In vitro and cellular assays demonstrate that Aurora-B phosphorylates vimentin at the cleavage furrow, indicating a critical role in intermediate filament disassembly during cytokinesis.

Key Points

To determine whether Aurora-B mediates cleavage furrow-specific phosphorylation of vimentin and to assess its functional role in intermediate filament segregation during cytokinesis.
Tracked the spatiotemporal localization of vimentin Ser-72 phosphorylation relative to Aurora-B from anaphase to telophase in cells with wild-type or dominant-negative Aurora-B.
Performed in vitro kinase assays to quantify Aurora-B phosphorylation of vimentin and evaluate its direct inhibitory effect on filament assembly.
Identified eight specific phosphorylation sites on vimentin and expressed an alanine-substituted mutant in dividing cells to assess division phenotypes.
Vimentin Ser-72 phosphorylation occurred selectively at the border of Aurora-B localization from anaphase to telophase, and dominant-negative Aurora-B expression reduced this phosphorylation.
Aurora-B phosphorylated vimentin at approximately 2 mol phosphate per mol substrate within 30 minutes in vitro, dramatically inhibiting filament formation.
Cells expressing the eight-site alanine vimentin mutant developed a persistent bridge-like intermediate filament structure between unseparated daughter cells.

Cite This Study

Goto et al. (2003) studied this question.

synapsesocial.com/papers/69da7cd684371aa676a3d366 https://doi.org/10.1074/jbc.m210892200

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Implication

In vitro and cellular assays demonstrate that Aurora-B phosphorylates vimentin at the cleavage furrow, indicating a critical role in intermediate filament disassembly during cytokinesis.

Aurora-B Regulates the Cleavage Furrow-specific Vimentin Phosphorylation in the Cytokinetic Process

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Cite This Study

Aurora-B Regulates the Cleavage Furrow-specific Vimentin Phosphorylation in the Cytokinetic Process

Discussion

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Implication

Key Points

Cite This Study

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