What question did this study set out to answer?

Identify the genetic basis of low cadmium accumulation in ion-beam-irradiated rice mutants and develop tools for breeding safer cultivars.

November 6, 2012Proceedings of the National Academy of SciencesOpen Access

Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice

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Authors

SISatoru IshikawaNational Agriculture and Food Research Organization YIYasuhiro IshimaruTohoku University MIMasato IguraNational Institute for Agro-Environmental Sciences

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Implication

Genetic mutagenesis study reveals marked cadmium reduction in rice grains via OsNRAMP5 mutations, highlighting a practical non-transgenic approach for safer food production.

Key Points

Identify the genetic basis of low cadmium accumulation in ion-beam-irradiated rice mutants and develop tools for breeding safer cultivars.
Irradiated Koshihikari rice seeds with carbon ion beams to isolate cadmium-deficient mutants.
Conducted sequencing and functional analyses to identify mutations in the OsNRAMP5 transporter gene and engineered specific DNA markers for selection.
Tested mutant lines in cadmium-contaminated paddy fields to monitor grain cadmium concentration and evaluate agronomic performance.
Three mutant strains accumulated <0.05 mg Cd⋅kg⁻¹ in the grain compared with 1.73 mg Cd⋅kg⁻¹ in the wild-type Koshihikari parent.
Defective transporter proteins encoded by mutated osnramp5 substantially reduced cadmium uptake through the roots without causing agriculturally or economically adverse traits.

Cite This Study

Ishikawa et al. (2012) studied this question.

synapsesocial.com/papers/69dab40eaae38ff6ad835ecf https://doi.org/10.1073/pnas.1211132109

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Implication

Genetic mutagenesis study reveals marked cadmium reduction in rice grains via OsNRAMP5 mutations, highlighting a practical non-transgenic approach for safer food production.

Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice

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Key Points

Cite This Study

Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice

Discussion

Member takes

Implication

Key Points

Cite This Study

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