What question did this study set out to answer?

To establish a sensitive, genome-wide qPCR profiling platform and quality control framework for evaluating circulating microRNAs across diverse biofluid samples.

October 1, 2012MethodsOpen Access

Assessing sample and miRNA profile quality in serum and plasma or other biofluids

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Authors

TBThórarinn BlöndalReykjavík University SNSøren Jensby NielsenAmgen (United States)ABAdam BakerUniversity Hospitals Coventry and Warwickshire NHS Trust

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Implication

Methodological study demonstrates quality control strategies for circulating microRNA in biofluids, highlighting reference ranges and hemolysis detection to improve biomarker discovery.

Key Points

To establish a sensitive, genome-wide qPCR profiling platform and quality control framework for evaluating circulating microRNAs across diverse biofluid samples.
Engineered a Locked Nucleic Acid (LNA)-based qPCR platform capable of high-throughput profiling of microRNAs directly from clinical biofluids without pre-amplification.
Profiled thousands of biofluid specimens, including blood-derived serum and plasma, to evaluate pre-analytical and analytical variables.
Established multi-step sample, RNA, and data quality control pipelines alongside normalization methods.
Achieved high-throughput, sensitive quantification of circulating microRNAs directly from plasma and serum without requiring pre-amplification steps.
Established baseline normal reference ranges for circulating microRNA expression patterns across serum and plasma samples.
Formulated an expression-based microRNA biomarker assay to detect and correct for sample hemolysis.

Cite This Study

Blöndal et al. (2012) studied this question.

synapsesocial.com/papers/69dac79ee32a2a6b95a3c031 https://doi.org/10.1016/j.ymeth.2012.09.015

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Implication

Methodological study demonstrates quality control strategies for circulating microRNA in biofluids, highlighting reference ranges and hemolysis detection to improve biomarker discovery.

Assessing sample and miRNA profile quality in serum and plasma or other biofluids

Discussion

Member takes

Implication

Key Points

Cite This Study

Assessing sample and miRNA profile quality in serum and plasma or other biofluids

Discussion

Member takes

Implication

Key Points

Cite This Study

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