What question did this study set out to answer?

To determine the molecular expression and mechanosensory role of the Piezo1 channel in bladder urothelial cells during mechanical stretch.

April 23, 2014Journal of Biological ChemistryOpen Access

Functional Role for Piezo1 in Stretch-evoked Ca2+ Influx and ATP Release in Urothelial Cell Cultures

View Full Paper

Ask AI

Bookmark

Authors

TMTatsuya MiyamotoMatsue Red Cross Hospital TMTsutomu MochizukiUniversity of Yamanashi HNHiroshi NakagomiBhabha Atomic Research Centre

Discussion

Member takes

Overview

Cell culture study reveals Piezo1 drives stretch-induced calcium influx and ATP release in urothelial cells, suggesting a novel therapeutic target for bladder dysfunction.

Key Points

To determine the molecular expression and mechanosensory role of the Piezo1 channel in bladder urothelial cells during mechanical stretch.
Evaluated Piezo1 protein expression in mouse and human bladder urothelium using immunofluorescence assays.
Measured intracellular calcium influx and ATP release in cultured mouse urothelial cells exposed to mechanical stretch, with and without Piezo1 knockdown or the channel inhibitor GsMTx4.
Piezo1 was abundantly expressed in both mouse and human bladder urothelium.
Mechanical stretch induced cytosolic calcium influx and subsequent ATP release in urothelial cells, both of which were suppressed by Piezo1 knockdown or GsMTx4 treatment.
Piezo1 and TRPV4 responded differentially to distinguish varying intensities of mechanical stretch stimulation.

Cite This Study

Miyamoto et al. (2014) studied this question.

synapsesocial.com/papers/69db06da498b35d3e6a3c253 https://doi.org/10.1074/jbc.m113.528638

View Full Paper

Ask AI

Bookmark

April 23, 2014Journal of Biological ChemistryOpen Access

Functional Role for Piezo1 in Stretch-evoked Ca2+ Influx and ATP Release in Urothelial Cell Cultures

View Full Paper

Ask AI

Bookmark

Authors

TMTatsuya MiyamotoMatsue Red Cross Hospital TMTsutomu MochizukiUniversity of Yamanashi HNHiroshi NakagomiBhabha Atomic Research Centre

Discussion

Member takes

Overview

Cell culture study reveals Piezo1 drives stretch-induced calcium influx and ATP release in urothelial cells, suggesting a novel therapeutic target for bladder dysfunction.

Key Points

To determine the molecular expression and mechanosensory role of the Piezo1 channel in bladder urothelial cells during mechanical stretch.
Evaluated Piezo1 protein expression in mouse and human bladder urothelium using immunofluorescence assays.
Measured intracellular calcium influx and ATP release in cultured mouse urothelial cells exposed to mechanical stretch, with and without Piezo1 knockdown or the channel inhibitor GsMTx4.
Piezo1 was abundantly expressed in both mouse and human bladder urothelium.
Mechanical stretch induced cytosolic calcium influx and subsequent ATP release in urothelial cells, both of which were suppressed by Piezo1 knockdown or GsMTx4 treatment.
Piezo1 and TRPV4 responded differentially to distinguish varying intensities of mechanical stretch stimulation.

Cite This Study

Miyamoto et al. (2014) studied this question.

synapsesocial.com/papers/69db06da498b35d3e6a3c253 https://doi.org/10.1074/jbc.m113.528638

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark