What question did this study set out to answer?

To determine the mechanism by which systemically administered adult mesenchymal stem/stromal cells (MSCs) ameliorate chemical injury to the cornea.

August 11, 2011Stem Cells

Action at a Distance: Systemically Administered Adult Stem/Progenitor Cells (MSCs) Reduce Inflammatory Damage to the Cornea Without Engraftment and Primarily by Secretion of TNF-α Stimulated Gene/Protein 6

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Authors

GRGavin W. RoddyMayo Clinic JOJoo Youn OhSeoul National University of Education RLRyang Hwa LeeTexas A&M University System

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Implication

Preclinical study demonstrates reduced corneal inflammation following systemic stem cell therapy in rats, indicating therapeutic effects occur via remote TSG-6 secretion without cell engraftment.

Key Points

To determine the mechanism by which systemically administered adult mesenchymal stem/stromal cells (MSCs) ameliorate chemical injury to the cornea.
Administered 1 × 10^7 human MSCs (hMSCs) intravenously (i.v.) or intraperitoneally (i.p.) to chemically injured rat corneas and quantified corneal engraftment using a human GAPDH mRNA assay at days 1 and 3.
Evaluated TSG-6 expression in vitro using transwell cocultures of injured corneal epithelial cells and hMSCs.
Tested the therapeutic necessity of TSG-6 in vivo via siRNA knockdown in hMSCs and assessed whether i.v. or topical recombinant TSG-6 alone duplicated MSC benefits.
Fewer than 10 hMSCs were present in the injured rat corneas 1 and 3 days after systemic administration, despite marked reductions in corneal inflammation and opacity.
Injured corneal epithelial cells activated hMSCs to secrete TSG-6 in vitro.
Knockdown of TSG-6 largely abolished the therapeutic efficacy of systemic hMSCs in vivo, whereas systemic or topical TSG-6 administration alone fully duplicated the therapeutic effect.

Cite This Study

Roddy et al. (2011) studied this question.

synapsesocial.com/papers/69db23bf0d8d6ef495a3d19a https://doi.org/10.1002/stem.708

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Implication

Preclinical study demonstrates reduced corneal inflammation following systemic stem cell therapy in rats, indicating therapeutic effects occur via remote TSG-6 secretion without cell engraftment.

Action at a Distance: Systemically Administered Adult Stem/Progenitor Cells (MSCs) Reduce Inflammatory Damage to the Cornea Without Engraftment and Primarily by Secretion of TNF-α Stimulated Gene/Protein 6

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Key Points

Cite This Study

Action at a Distance: Systemically Administered Adult Stem/Progenitor Cells (MSCs) Reduce Inflammatory Damage to the Cornea Without Engraftment and Primarily by Secretion of TNF-α Stimulated Gene/Protein 6

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