Exercise induces transient transcriptional activation of the PGC‐1α gene in human skeletal muscle
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Implication
Experimental study demonstrates transient PGC-1α transcriptional activation in human skeletal muscle after exercise, suggesting its key role in coordinating metabolic gene expression.
Key Points
- To determine whether acute exercise and endurance exercise training regulate PGC-1α transcription and downstream metabolic gene expression in human skeletal muscle.
- Seven male subjects completed 4 weeks of single-leg knee extensor exercise training, followed by an acute 3-hour bout of two-legged knee extensor exercise.
- Vastus lateralis muscle biopsies were obtained from both the trained and untrained legs prior to exercise and after 0, 2, 6, and 24 hours of recovery.
- Acute exercise induced a marked, transient increase (P < 0.05) in PGC-1α transcription (10- to > 40-fold) and mRNA content (7- to 10-fold), peaking within 2 hours of recovery, with greater activation observed in the trained leg.
- NRF-1 mRNA levels remained unchanged post-exercise, whereas hexokinase II, mitochondrial transcription factor A, PPAR-α, and calcineurin Aα/Aβ mRNA elevated 2- to 6-fold (P < 0.05) at 6 hours of recovery exclusively in the untrained leg.
Cite This Study
Pilegaard et al. (2003) studied this question.