ABSTRACT Numerous methods involving reducing and stabilizing agents were applied in the past to produce silver nanoparticles (AgNPs) rapidly. However, these reducing agents are unsafe thus using a green approach over chemical techniques is a need for today as they are ecofriendly, low‐cost, sustainable, and biocompatible. Therefore, the AgNPs were produced using Aspergillus fumigatus BTCC10 (KY486782) by addition of various reducing and capping agents like ethylene diamine tetra acetic acid, glucose, polyethylene glycol, ammonium sulfate, glycine, and cellulase. These common agents were added to the extract and their effects were examined on the morphology of the nanoparticles (NPs). AgNPs with varying shapes and sizes were formed from spherical to irregular shape which were later confirmed by atomic force microscopy; whereas the size of NPs produced fluctuated from 33.45 to 255.7 nm. The absorption peaks were detected between 398 and 465 nm for these added reducing and enhancing agents verifying the establishment of NPs. The results showed that AgNPs by the supplementation of cellulase (5%) developed the smallest sized nanoparticles (33.45 ± 0.08 nm) without aggregation with zeta potential of −7.40 mV along with X‐ray diffraction studies authenticating its crystalline characteristics that is significant. Henceforth its antibacterial activity was analyzed against Pseudomonas aeruginosa (BTCB01), Staphylococcus aureus (BTCB02), and Escherichia coli (BTCB03) through disc diffusion method. Later, zone of inhibition was evaluated against E. coli at 24 ± 0.30 mm which is noteworthy. The NPs demonstrated effective antibacterial activity with microbes that can benefit many sectors like pharmaceutical, textile, food and cosmetics in future. In the end, the yield (%) of the generated AgNPs was also calculated and the sample with addition of cellulase contributed towards a maximum yield of 52.6 ± 1.08% that is unique.
Shahzad et al. (Fri,) studied this question.