Microfluidic toolbox using padlock probes and rolling circle amplification for direct detection and genotyping of viral RNA

João Varela; Priscilla Gomes da Silva; Hower Lee; João R. Mesquita; Aman Russom; Ruben R. G. Soares; Mats Nilsson

doi:10.1039/d6ra00912c

January 1, 2026RSC AdvancesOpen Access

Microfluidic toolbox using padlock probes and rolling circle amplification for direct detection and genotyping of viral RNA

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Key Points

To evaluate the effectiveness of rolling circle amplification combined with padlock probes for viral RNA detection and genotyping.
Utilized rolling circle amplification (RCA) for amplification of viral RNA.
Employed padlock probes to enhance specificity in detecting viral genetic material.
Compared the efficacy of this method with traditional RT-PCR techniques.
Demonstrated higher sensitivity in detecting viral RNA compared to RT-PCR.
Showed potential for faster results in genotyping of viral samples.
Indicated that this method could reduce costs and simplify the diagnostic process.

Implication

This research demonstrates direct detection and genotyping of viral RNA, suggesting a novel tool that may improve diagnostic accuracy.

Abstract

Rolling circle amplification (RCA) combined with padlock probes presents a promising tool for direct detection and genotyping of viral RNA, offering advantages over conventional methods like RT-PCR.

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Cite This Study

Varela et al. (2026) studied this question.

synapsesocial.com/papers/69db38534fe01fead37c6927 https://doi.org/10.1039/d6ra00912c

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Microfluidic toolbox using padlock probes and rolling circle amplification for direct detection and genotyping of viral RNA

Key Points

Implication

Abstract

Discussion

Cite This Study

Microfluidic toolbox using padlock probes and rolling circle amplification for direct detection and genotyping of viral RNA

Key Points

Implication

Abstract

Discussion

Cite This Study

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