What question did this study set out to answer?

To determine the in vivo functional role of OX40 ligand and its interaction with OX40 in regulating antigen-presenting cell function and T-cell activation.

January 17, 2000The Journal of Experimental MedicineOpen Access

Impairment of Antigen-Presenting Cell Function in Mice Lacking Expression of Ox40 Ligand

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To determine the in vivo functional role of OX40 ligand and its interaction with OX40 in regulating antigen-presenting cell function and T-cell activation.
Generated OX40L-deficient mice and an antagonistic anti-OX40L monoclonal antibody (MGP34).
Stimulated splenic B cells with CD40 and anti-IgM, and dendritic cells with CD40 ligation alone, to analyze OX40L surface induction.
Evaluated antigen-specific in vivo immune responses using protein antigens, alloantigens, and keyhole limpet hemocyanin immunizations.
OX40L-deficient and MGP34-treated mice exhibited marked suppression of T-cell recall responses to protein and alloantigens along with a significant reduction in keyhole limpet hemocyanin-specific IgG production.
Loss of OX40L impaired intrinsic antigen-presenting cell function during both the priming and effector phases of T-cell activation, with a concomitant decrease in both Th1 and Th2 cytokine levels.

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Implication

Controlled mouse study demonstrates impaired T-cell priming and antibody responses in OX40L deficiency, indicating OX40L on antigen-presenting cells is critical for in vivo immunity.

Cite This Study

Murata et al. (2000) studied this question.

synapsesocial.com/papers/69db3a1f0d8d6ef495a3d3f9 https://doi.org/10.1084/jem.191.2.365

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Implication

Controlled mouse study demonstrates impaired T-cell priming and antibody responses in OX40L deficiency, indicating OX40L on antigen-presenting cells is critical for in vivo immunity.

Impairment of Antigen-Presenting Cell Function in Mice Lacking Expression of Ox40 Ligand

Key Points

Discussion

Implication

Cite This Study

Impairment of Antigen-Presenting Cell Function in Mice Lacking Expression of Ox40 Ligand

Key Points

Discussion

Implication

Cite This Study

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