What question did this study set out to answer?

To measure the cellular DNA content and determine the ploidy status of Cryptococcus neoformans strains isolated from clinical and environmental sources.

January 1, 1996Medical MycologyOpen Access

Determination of ploidy inCryptococcus neoformansby flow cytometry

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Authors

RTReiko TanakaImperial College London HTHideaki TaguchiChiba University Hospital KTKanji TakeoChiba University

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Implication

Flow cytometric analysis demonstrates natural diploidy in Cryptococcus neoformans isolates, indicating widespread genetic variation in fungal ploidy.

Key Points

To measure the cellular DNA content and determine the ploidy status of Cryptococcus neoformans strains isolated from clinical and environmental sources.
Stained 28 clinical and environmental isolates alongside two reference haploid strains using propidium iodide.
Assessed cellular DNA content and fluorescence intensity distributions using flow cytometry.
Authentic haploid reference strains and 23 of 28 tested isolates exhibited diphasic histograms with peaks centered at channels 9 and 18.
Five of 28 isolates displayed peaks shifted to channels 18 and 35, exhibiting double the cellular DNA content of haploid controls and confirming diploidy.

Cite This Study

Tanaka et al. (1996) studied this question.

synapsesocial.com/papers/69db4ced74ec163421835a09 https://doi.org/10.1080/02681219680000521

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Implication

Flow cytometric analysis demonstrates natural diploidy in Cryptococcus neoformans isolates, indicating widespread genetic variation in fungal ploidy.

Determination of ploidy inCryptococcus neoformansby flow cytometry

Discussion

Member takes

Implication

Key Points

Cite This Study

Determination of ploidy inCryptococcus neoformansby flow cytometry

Discussion

Member takes

Implication

Key Points

Cite This Study

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