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Integrate in-skull magnetic resonance histology with whole-brain light sheet microscopy to create a high-throughput, distortion-corrected platform for quantitative mouse neurogenetics.

October 9, 2021Open Access

Multimodal Magnetic Resonance Histology and Light Sheet Imaging for Quantitative Neurogenetics of the Mouse

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Integrate in-skull magnetic resonance histology with whole-brain light sheet microscopy to create a high-throughput, distortion-corrected platform for quantitative mouse neurogenetics.
Acquired in-skull magnetic resonance histology (MRH) at 15 μm isotropic resolution with 5 μm super-resolution track density imaging to establish undistorted brain geometry and connectivity.
Conducted multichannel light sheet microscopy (LSM) at 1.8 × 1.8 × 4.0 μm resolution on cleared, immunostained mouse brains within scan times under six hours.
Implemented a computational image processing pipeline to correct LSM geometric distortions using MRH references, transfer atlas labels, and quantify regional cell densities.
Achieved an MRH resolution index of 32 million, providing comprehensive whole-brain structural connectomes in less than one day.
Overcame light sheet tissue distortion, swelling, and damage by registering cleared tissue volumes directly to intact-skull MRH coordinate frames.
Successfully extracted standardized image-derived phenotypes of cellular distributions and circuit architecture across the entire mouse brain.

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Implication

Methodological study demonstrates integrated magnetic resonance histology and light sheet imaging in mice, highlighting high-throughput mapping of neural circuits.

Cite This Study

Johnson et al. (2021) studied this question.

synapsesocial.com/papers/69db9db6c9a120f055a3c217 https://doi.org/10.1101/2021.10.08.463660

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Implication

Methodological study demonstrates integrated magnetic resonance histology and light sheet imaging in mice, highlighting high-throughput mapping of neural circuits.

Multimodal Magnetic Resonance Histology and Light Sheet Imaging for Quantitative Neurogenetics of the Mouse

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Implication

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Multimodal Magnetic Resonance Histology and Light Sheet Imaging for Quantitative Neurogenetics of the Mouse

Key Points

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Implication

Cite This Study

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