Nanoparticles Coated with High Molecular Weight PEG Penetrate Mucus and Provide Uniform Vaginal and Colorectal Distribution in Vivo

We demonstrate here that PEG with MW as high as 40 kDa can be densely grafted to the surface of NP to prevent interactions with mucus. NP coated with 10-40 kDa PEG rapidly diffused through human cervicovaginal mucus ex vivo, and uniformly lined the mouse colorectal and vaginal epithelium in vivo.