What question did this study set out to answer?

To determine whether oxidative stress markers and antioxidant defenses change across progressive stages of Alzheimer disease in subcellular fractions of the human frontal cortex.

January 21, 2010Journal of Neuropathology & Experimental NeurologyOpen Access

Oxidative Stress in the Progression of Alzheimer Disease in the Frontal Cortex

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Authors

MAMubeen A. AnsariSUNY Upstate Medical University SSStephen W. ScheffUniversity of Kentucky

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Implication

Postmortem study reveals progressive synaptic oxidative stress and antioxidant loss across Alzheimer stages, implicating localized oxidative damage in disease-related synaptic loss.

Key Points

To determine whether oxidative stress markers and antioxidant defenses change across progressive stages of Alzheimer disease in subcellular fractions of the human frontal cortex.
Analyzed postmortem frontal cortex tissue from subjects with no cognitive impairment (n=10), mild cognitive impairment (n=8), mild/moderate Alzheimer disease (n=4), and late-stage Alzheimer disease (n=9).
Separated brain tissue with short postmortem intervals into postmitochondrial supernatant, nonsynaptic mitochondria, and synaptosome fractions.
Measured antioxidant levels (glutathione, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, glucose-6-phosphate dehydrogenase, superoxide dismutase, catalase) and oxidative damage markers (TBARS, protein carbonyls, 3-nitrotyrosine, 4-hydroxynonenal, acrolein).
All three neuropil fractions demonstrated significant disease-dependent increases in oxidative stress markers, with the largest increases localized to synaptosomes.
Mitochondrial and synaptosomal fractions exhibited significant declines in antioxidants, specifically glutathione, glutathione peroxidase, glutathione-S-transferase, and superoxide dismutase.
Oxidative marker levels correlated significantly with Mini-Mental Status Examination scores, indicating localized synaptic oxidative stress that intensifies with clinical disease progression.

Cite This Study

Ansari et al. (2010) studied this question.

synapsesocial.com/papers/69dbdfb9e6ab964fb0837139 https://doi.org/10.1097/nen.0b013e3181cb5af4

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Implication

Postmortem study reveals progressive synaptic oxidative stress and antioxidant loss across Alzheimer stages, implicating localized oxidative damage in disease-related synaptic loss.

Oxidative Stress in the Progression of Alzheimer Disease in the Frontal Cortex

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Cite This Study

Oxidative Stress in the Progression of Alzheimer Disease in the Frontal Cortex

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Implication

Key Points

Cite This Study

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