What question did this study set out to answer?

To screen and identify high-affinity monoclonal antibody pairs targeting urinary lipoarabinomannan to improve the diagnostic sensitivity and specificity of tuberculosis testing.

September 24, 2018Journal of Clinical MicrobiologyOpen Access

A Novel Sensitive Immunoassay Targeting the 5-Methylthio- d -Xylofuranose–Lipoarabinomannan Epitope Meets the WHO's Performance Target for Tuberculosis Diagnosis

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To screen and identify high-affinity monoclonal antibody pairs targeting urinary lipoarabinomannan to improve the diagnostic sensitivity and specificity of tuberculosis testing.
Screened 100 monoclonal antibody pairs targeting lipoarabinomannan (LAM) epitopes on an electrochemiluminescence platform.
Evaluated the 12 best-performing antibody pairs in a retrospective case-control study using urine samples from 75 adults with presumptive tuberculosis.
The lead antibody pair achieved an overall clinical sensitivity of 93% (95% CI, 80% to 97%) and specificity of 97% (95% CI, 85% to 100%), compared to a sensitivity of 33% (95% CI, 20% to 48%) for the commercial Alere LF-LAM assay.
In HIV-negative subjects with smear-positive tuberculosis, the assay achieved 80% sensitivity (95% CI, 55% to 93%) compared to 13% (95% CI, 4% to 38%) for Alere LF-LAM.
The capture antibody specifically binds a 5-methylthio-d-xylofuranose (MTX)-dependent epitope unique to Mycobacterium tuberculosis complex, exhibiting no cross-reactivity with fast-growing mycobacteria or other bacterial species.

Authors

GSGeorge B. SigalMeso Scale Discovery (United States)APAbraham PinterRutgers, The State University of New Jersey TLTodd L. LowaryUniversity of Alberta

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Implication

Retrospective case-control study reveals high diagnostic accuracy of a novel urine lipoarabinomannan test in adults with presumptive tuberculosis, indicating viable point-of-care detection.

Cite This Study

Sigal et al. (2018) studied this question.

synapsesocial.com/papers/69dbeaf7c34c3c3eb4684151 https://doi.org/10.1128/jcm.01338-18

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Implication

Retrospective case-control study reveals high diagnostic accuracy of a novel urine lipoarabinomannan test in adults with presumptive tuberculosis, indicating viable point-of-care detection.

A Novel Sensitive Immunoassay Targeting the 5-Methylthio- d -Xylofuranose–Lipoarabinomannan Epitope Meets the WHO's Performance Target for Tuberculosis Diagnosis

Key Points

Discussion

Implication

Cite This Study

A Novel Sensitive Immunoassay Targeting the 5-Methylthio- d -Xylofuranose–Lipoarabinomannan Epitope Meets the WHO's Performance Target for Tuberculosis Diagnosis

Key Points

Discussion

Implication

Cite This Study

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