What question did this study set out to answer?

To establish a sensitive method for measuring local microviscosity in living biological systems using a BODIPY-based fluorescent molecular rotor.

Molecular Rotor Measures Viscosity of Live Cells via Fluorescence Lifetime Imaging

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Fluorescence imaging demonstrates high microviscosity in live cells, suggesting a structural constraint on the diffusion of reactive oxygen species.

To establish a sensitive method for measuring local microviscosity in living biological systems using a BODIPY-based fluorescent molecular rotor.
Incubated live cells with the fluorescent molecular rotor 4,4'-difluoro-4-bora-5-(p-oxoalkyl)phenyl-3a,4a-diaza-s-indacene.
Quantified microviscosity via fluorescence lifetime imaging (FLIM) and evaluated intracellular binding using time-resolved fluorescence anisotropy.
Recorded a distinct intracellular rotor lifetime of 1.6 ± 0.2 ns, corresponding to an intracellular microviscosity of approximately 140 cP.
Observed an intracellular viscosity substantially higher than that of water and bulk cytoplasm, with time-resolved anisotropy confirming negligible intracellular fluorophore binding.

Kuimova et al. (2008) studied this question.

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Fluorescence imaging demonstrates high microviscosity in live cells, suggesting a structural constraint on the diffusion of reactive oxygen species.