What question did this study set out to answer?

The aim is to clone and characterize a novel estrogen receptor in rats and investigate its expression in tissues.

Cloning of a novel receptor expressed in rat prostate and ovary.

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Randomized trial explores a new estrogen receptor in rat prostate and ovary, suggesting implications for hormonal research.

The aim is to clone and characterize a novel estrogen receptor in rats and investigate its expression in tissues.
Isolated cDNA from rat prostate library to clone a nuclear receptor protein.
Conducted in situ hybridization to study expression in prostate and ovary tissues.
Performed ligand-binding analysis to assess binding affinity for various hormones.
Clone 29 protein shows 95% homology with rat estrogen receptor in DNA-binding domain.
17beta-estradiol binds with high affinity (Kd=0.6 nM) to clone 29.
Maximal stimulation of reporter gene activity was observed with 10 nM E2, reaching about 3-fold increase.

Kuiper et al. (1996) studied this question.

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