What question did this study set out to answer?

To overcome stress-induced hyphal transition and maximize β-carotene biosynthesis in the dimorphic oleaginous yeast Yarrowia lipolytica.

November 11, 2021ACS Synthetic Biology

Morphological and Metabolic Engineering of Yarrowia lipolytica to Increase β-Carotene Production

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To overcome stress-induced hyphal transition and maximize β-carotene biosynthesis in the dimorphic oleaginous yeast Yarrowia lipolytica.
Integrated multiple copies of 13 genes involved in the β-carotene biosynthesis pathway into Y. lipolytica.
Deleted the morphological regulatory genes CLA4 and MHY1 to revert stress-induced hyphae back into the favorable yeast form.
Assessed β-carotene titer and cellular content under fed-batch fermentation conditions.
Multigene pathway integration increased β-carotene content 11.7-fold relative to start strain T1 but triggered a morphological shift from oval yeast to hyphae.
Knockout of CLA4 and MHY1 restored yeast morphology and increased β-carotene production by an additional 139%.
Fed-batch fermentation of the optimized strain achieved a yield of 159 mg/g dry cell weight and a titer of 7.6 g/L.

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Implication

Experimental engineering demonstrates record beta-carotene yield in Yarrowia lipolytica through dimorphism suppression, highlighting morphological control as a viable bioproduction strategy.

Cite This Study

Liu et al. (2021) studied this question.

synapsesocial.com/papers/69dc58a5d74bf23813c0fe7e https://doi.org/10.1021/acssynbio.1c00480

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Implication

Experimental engineering demonstrates record beta-carotene yield in Yarrowia lipolytica through dimorphism suppression, highlighting morphological control as a viable bioproduction strategy.

Morphological and Metabolic Engineering of Yarrowia lipolytica to Increase β-Carotene Production

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Discussion

Implication

Cite This Study

Morphological and Metabolic Engineering of Yarrowia lipolytica to Increase β-Carotene Production

Key Points

Discussion

Implication

Cite This Study

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