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April 13, 2026Plant Cell Tissue and Organ Culture (PCTOC)Open Access

Optimization of in vitro propagation of blueberry: influence of plant growth regulators and medium components on multiplication and rooting

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Authors

MRMariana de Souza RibeiroUniversidade Federal de LavrasFRFilipe Almendagna RodriguesUniversidade Federal de LavrasRARonilson Carlos AraújoUniversidade Federal de Lavras

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Overview

The study optimizes in vitro propagation and rooting in blueberry cultivars, implying improved production efficiency.

Key Points

  • The aim is to enhance shoot multiplication rates and rooting efficiency for blueberry cultivars under in vitro conditions.
  • Cultured stem segments on Woody Plant Medium (WPM) with varying growth regulators.
  • Evaluated combinations of 2-isopentenyladenine (2-ip) and adenine sulfate for shoot multiplication.
  • Microcuttings were inoculated with indole-3-butyric acid (IBA) during the rooting phase.
  • Different concentrations of WPM and activated charcoal were tested for rooting efficiency.
  • Combination of 2.5 mg L⁻¹ 2-ip with 10 mg L⁻¹ adenine sulfate resulted in maximum shoot proliferation.
  • Woodard responded best to 2.5 mg L⁻¹ 2-ip and 20 mg L⁻¹ adenine sulfate.
  • Bluegem achieved optimal rooting at 75% WPM.
  • Activated charcoal improved rooting only for Climax.
  • Overall improvement in ex vitro survival rates and acclimatization efficiency.

Cite This Study

Ribeiro et al. (2026) studied this question.

synapsesocial.com/papers/69dc87ea3afacbeac03e9f9ehttps://doi.org/10.1007/s11240-026-03440-z
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