What question did this study set out to answer?

To identify the specific sites of macromolecular transport within the nuclear envelope and determine whether molecular size limits the nuclear entry of karyophilic proteins.

December 1, 1984The Journal of Cell BiologyOpen Access

Movement of a karyophilic protein through the nuclear pores of oocytes.

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Authors

CFCarl M. FeldherrUniversity of Florida EKErnst KallenbachTechnische Universität Ilmenau NSNadine SchultzUniversity of Massachusetts Chan Medical School

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Implication

Experimental study demonstrates that nucleoplasmin mediates nuclear pore transport of particles up to 200 angstroms in amphibian oocytes, indicating specific signal-dependent translocation.

Key Points

To identify the specific sites of macromolecular transport within the nuclear envelope and determine whether molecular size limits the nuclear entry of karyophilic proteins.
Coated colloidal gold particles (diameter 20 to 170 Å) with intact nucleoplasmin, trypsin-digested nucleoplasmin, or polyvinylpyrrolidone.
Microinjected the coated particles into the cytoplasm of Xenopus oocytes and fixed the cells at 15 minutes and 1 hour post-injection.
Tracked the intracellular localization and comparative size distributions of gold particles using electron microscopy.
Nucleoplasmin-coated colloidal gold readily crossed the nuclear envelope specifically by passing through nuclear pores.
Particle size distributions in the nucleus and cytoplasm did not differ significantly, with the envelope accommodating total particle diameters between 50 and 200 Å.
Particles coated with trypsin-digested nucleoplasmin or polyvinylpyrrolidone were largely excluded from the nucleus and exhibited no transport.

Cite This Study

Feldherr et al. (1984) studied this question.

synapsesocial.com/papers/69dc926324e766dc31359613 https://doi.org/10.1083/jcb.99.6.2216

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Implication

Experimental study demonstrates that nucleoplasmin mediates nuclear pore transport of particles up to 200 angstroms in amphibian oocytes, indicating specific signal-dependent translocation.

Movement of a karyophilic protein through the nuclear pores of oocytes.

Discussion

Member takes

Implication

Key Points

Cite This Study

Movement of a karyophilic protein through the nuclear pores of oocytes.

Discussion

Member takes

Implication

Key Points

Cite This Study

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