A stable iron‐hæmatoxylin solution for staining the chromatin of cell nuclei

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SUMMARY By using ferrous sulphate as mordant, sodium iodate as oxidant and aluminium chloride as stabilizer, it proved possible to formulate a stable single‐solution iron‐hæmatoxylin. This provides a resistant nuclear stain, sufficiently acid‐fast to be suitable for counterstaining with van Gieson or the trichrome methods. This solution, ferrous hæmatein, can replace the celestin blue‐hæmalum sequence.