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March 1, 2006Foodborne Pathogens and Disease

Standardization of Pulsed-Field Gel Electrophoresis Protocols for the Subtyping of Escherichia coli O157:H7, Salmonella , and Shigella for PulseNet

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Authors

EREfrain M. RibotCenters for Disease Control and PreventionMFM. A. Lambert FairCenters for Disease Control and PreventionRGRomesh GautomWashington State Department of Health

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Implication

Methodological standardization demonstrates rapid 24-hour subtyping of bacterial foodborne pathogens across public health laboratories, facilitating early detection of outbreak clusters.

Key Points

  • Standardize rapid pulsed-field gel electrophoresis (PFGE) protocols for subtyping Escherichia coli O157:H7, Salmonella, and Shigella species to support the PulseNet surveillance network.
  • Optimized reagents and reaction conditions based on an original 3- to 4-day CDC protocol validated in 1996 and 1997 across eight independent laboratories.
  • Implemented standardized testing conditions across local, state, and national public health laboratories participating in PulseNet.
  • Shortened the procedural time frame for bacterial subtyping from 3–4 days to approximately 24 hours.
  • Yielded reproducible, high-quality PFGE pattern data across participating laboratories to facilitate early cluster detection and outbreak source identification.

Cite This Study

Ribot et al. (2006) studied this question.

synapsesocial.com/papers/69dcc9c91e43378fbd133562https://doi.org/10.1089/fpd.2006.3.59
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Also Consider

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  1. 1Emerging Foodborne Diseases: An Evolving Public Health Challenge1997 · 638 citations
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  5. 5Laboratory investigation of a multistate food-borne outbreak of Escherichia coli O157:H7 by using pulsed-field gel electrophoresis and phage typing1994 · 346 citations