Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
June 1, 1990Applied and Environmental MicrobiologyOpen Access

Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations

View Full Paper
Ask AI
Bookmark
Share

Authors

RARudolf AmannBBBrian J. BinderRORobert Olson

Discussion

Loading...

Member takes

Overview

Experimental technique analyzes mixed microbial populations, enhancing fluorescence for better quantification.

Key Points

  • This research aims to develop a method for quantifying bacterial cells in mixed populations using fluorescent oligonucleotide probes and flow cytometry.
  • Fluorescent oligonucleotide hybridization probes labeled with tetramethylrhodamine were used.
  • Probes targeted short sequences within the 16S rRNA of microorganisms.
  • Flow cytometry resolved and quantified target and nontarget bacteria by their fluorescence.
  • Target cells were successfully quantified in a mixture with nontarget cells.
  • Using two or three different fluorescent probes enhanced the intensity of fluorescence.
  • Individual bacterial cells ranged from 1 to 5 microns in size.

Cite This Study

Amann et al. (1990) studied this question.

synapsesocial.com/papers/69dcf8fb7d2a65005be52d4ahttps://doi.org/10.1128/aem.56.6.1919-1925.1990
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Nucleotide sequence of the Dictyostelium discoideum small-subunit ribosomal ribonucleic acid inferred from the gene sequence: evolutionary implications1983 · 173 citations
  2. 2Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology1990 · 2,246 citations
  3. 3Natural relationship between bacteroides and flavobacteria1985 · 143 citations
  4. 4Flow cytometric detection of ribosomal RNA in suspended cells by fluorescent in situ hybridization1988 · 57 citations
  5. 5Phylogenetic Stains: Ribosomal RNA-Based Probes for the Identification of Single Cells1989 · 1,241 citations