What question did this study set out to answer?

Investigate the chemical stability of N-ethylmaleimide in solution and characterize its specific reaction kinetics with sulfhydryl (thiol) groups.

July 1, 1955Journal of the American Chemical Society

The Stability of N-Ethylmaleimide and its Reaction with Sulfhydryl Groups

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Investigate the chemical stability of N-ethylmaleimide in solution and characterize its specific reaction kinetics with sulfhydryl (thiol) groups.
Assessed the stability and degradation rates of N-ethylmaleimide across varying experimental conditions.
Measured the chemical reaction kinetics and binding specificity between N-ethylmaleimide and sulfhydryl-containing compounds.
Established the stability parameters of N-ethylmaleimide in aqueous systems.
Demonstrated rapid, selective covalent addition of N-ethylmaleimide across sulfhydryl groups.

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Implication

Biochemical analysis demonstrates the stability and reactivity of N-ethylmaleimide with sulfhydryl groups, highlighting its utility as a selective thiol-modifying reagent.

Cite This Study

John D. Gregory (1955) studied this question.

synapsesocial.com/papers/69dd480599c691022d99bb21 https://doi.org/10.1021/ja01619a073

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Implication

Biochemical analysis demonstrates the stability and reactivity of N-ethylmaleimide with sulfhydryl groups, highlighting its utility as a selective thiol-modifying reagent.

The Stability of N-Ethylmaleimide and its Reaction with Sulfhydryl Groups

Key Points

Discussion

Implication

Cite This Study

The Stability of N-Ethylmaleimide and its Reaction with Sulfhydryl Groups

Key Points

Discussion

Implication

Cite This Study

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