What question did this study set out to answer?

To evaluate mix-and-inject serial crystallography as a general tool for resolving the structural dynamics and kinetics of macromolecular reactions.

May 30, 2018BMC BiologyOpen Access

Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography

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Authors

JOJ.L. OlmosUniversity of California, San Francisco SPSuraj PandeyUniversity of Wisconsin–Milwaukee JMJosé M. Martín-GarcíaNational Research Council

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Implication

Methodological study demonstrates dynamic enzyme intermediate tracking via serial crystallography, highlighting applications for kinetics and structure-based drug design.

Key Points

To evaluate mix-and-inject serial crystallography as a general tool for resolving the structural dynamics and kinetics of macromolecular reactions.
Assessed mix-and-inject serial crystallography (MISC) for initiating biological macromolecule reactions prior to X-ray exposure.
Modeled high-speed data acquisition using megahertz X-ray pulse rates at European X-ray Free-Electron Laser and Linac Coherent Light Source II facilities.
Rapid collection across closely spaced time delays allows the extraction of both structural intermediates and reaction kinetics from unified X-ray datasets.
The approach provides a broadly applicable framework for characterizing dynamic reaction mechanisms crucial for structure-based drug design.

Cite This Study

Olmos et al. (2018) studied this question.

synapsesocial.com/papers/69de410eda08968cf7b0be9e https://doi.org/10.1186/s12915-018-0524-5

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Implication

Methodological study demonstrates dynamic enzyme intermediate tracking via serial crystallography, highlighting applications for kinetics and structure-based drug design.

Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography

Discussion

Member takes

Implication

Key Points

Cite This Study

Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography

Discussion

Member takes

Implication

Key Points

Cite This Study

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