Key Points
- To quantify crown-like structures reflecting dead adipocytes across visceral and subcutaneous fat depots in genetically obese mouse models.
- Quantified crown-like structures (CLS) and adipocyte size in visceral and subcutaneous fat depots of genetically obese db/db and ob/ob mice.
- Analyzed the relationship between adipocyte size and CLS density across fat depot locations.
- Crown-like structures were significantly more prevalent in visceral adipose depots compared to subcutaneous adipose depots across both db/db and ob/ob mice.
- Adipocyte size and CLS density correlated positively overall, but visceral fat exhibited the highest CLS density despite having smaller adipocyte sizes than subcutaneous fat.
- Visceral fat CLS density was 3.4-fold greater in db/db mice compared with ob/ob mice.
Structured PICO
PPopulationGenetically obese mice (db/db and ob/ob strains)
OOutcomeQuantification of crown-like structures (CLS) density and adipocyte size in visceral and subcutaneous fat depotssurrogate
Dead adipocytes forming crown-like structures are more prevalent in visceral than subcutaneous fat in genetically obese mice, potentially explaining the link between visceral adiposity and metabolic disorders.