What question did this study set out to answer?

To comprehensively map cellular lysine acetylation sites and assess how this modification regulates macromolecular protein complexes and biological pathways.

July 16, 2009ScienceOpen Access

Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions

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Authors

CCChunaram ChoudharyNovo Nordisk Foundation CKChanchal KumarVignana Jyothi Institute of Management FGFlorian GnadRoche (Switzerland)

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Implication

Proteomic profiling reveals extensive lysine acetylation across macromolecular complexes in cellular models, demonstrating regulatory scope comparable to other major protein modifications.

Key Points

To comprehensively map cellular lysine acetylation sites and assess how this modification regulates macromolecular protein complexes and biological pathways.
Employed high-resolution mass spectrometry to map global lysine acetylation sites and quantify acetylation dynamics.
Treated cells with deacetylase inhibitors suberoylanilide hydroxamic acid (SAHA) and MS-275 to measure induced changes in acetylation levels.
Identified 3,600 lysine acetylation sites on 1,750 proteins, showing significant enrichment on large complexes involved in chromatin remodeling, cell cycle, splicing, nuclear transport, and actin nucleation.
Demonstrated that lysine acetylation inhibits phosphorylation-dependent 14-3-3 protein interactions and regulates the activity of yeast cyclin-dependent kinase Cdc28.

Cite This Study

Choudhary et al. (2009) studied this question.

synapsesocial.com/papers/69de5c9d741e97d2d4e93a6f https://doi.org/10.1126/science.1175371

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Implication

Proteomic profiling reveals extensive lysine acetylation across macromolecular complexes in cellular models, demonstrating regulatory scope comparable to other major protein modifications.

Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions

Discussion

Member takes

Implication

Key Points

Cite This Study

Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions

Discussion

Member takes

Implication

Key Points

Cite This Study

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