What question did this study set out to answer?

To identify the biological function of the human epithelial glycoprotein EGP40 (Ep-CAM), a widely expressed tumor marker of unknown activity.

April 15, 1994The Journal of Cell BiologyOpen Access

Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule.

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To identify the biological function of the human epithelial glycoprotein EGP40 (Ep-CAM), a widely expressed tumor marker of unknown activity.
Transfected full-length human EGP40 cDNA into murine fibroblastic L cells and dedifferentiated mammary carcinoma L153S cells.
Assessed transfectants for calcium-dependent or calcium-independent homophilic adhesion, suspension aggregation, cell sorting in mixed populations, and invasive growth in EHS-matrigel.
EGP40 mediated homophilic cell-cell adhesion in a calcium-independent manner, increasing intercellular adhesion and driving aggregation in suspension assays.
Expression of EGP40 induced segregation of transfected cells from parental cells and suppressed invasive colony growth of L cells in EHS-matrigel by promoting tight intercellular adhesions.

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Implication

In vitro experimental study demonstrates that Ep-CAM mediates calcium-independent homophilic adhesion in murine cell lines, suggesting a key role in epithelial architecture.

Cite This Study

Litvinov et al. (1994) studied this question.

synapsesocial.com/papers/69de893d7ed287395e559865 https://doi.org/10.1083/jcb.125.2.437

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Implication

In vitro experimental study demonstrates that Ep-CAM mediates calcium-independent homophilic adhesion in murine cell lines, suggesting a key role in epithelial architecture.

Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule.

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Discussion

Implication

Cite This Study

Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule.

Key Points

Discussion

Implication

Cite This Study

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