What question did this study set out to answer?

To investigate transcriptomic alterations in young and senescent human dermal fibroblasts cultured within three-dimensional glycated type I collagen matrices over 10 and 17 days.

May 16, 2025International Journal of Molecular SciencesOpen Access

Changes in Gene Expression Patterns in Young and Senescent Fibroblasts in Glycated Three-Dimensional Collagen Matrices

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To investigate transcriptomic alterations in young and senescent human dermal fibroblasts cultured within three-dimensional glycated type I collagen matrices over 10 and 17 days.
Cultured young and senescent human dermal fibroblasts (HdFbs) in 3D collagen type I matrices post-glycated with varying doses of D-ribose.
Evaluated matrix stiffness, fluorescent advanced glycation end product (AGE) accumulation, and cell transcriptome profiles after 10 and 17 days of culture.
D-ribose treatment increased fluorescent AGE accumulation and matrix stiffness in a dose-dependent manner.
Transcriptomic profiling revealed activation of age-related genes and signaling pathways, including elements of the senescence-associated secretory phenotype (SASP).
Transcriptome alterations were more pronounced, exhibiting higher gene counts and larger fold changes, at day 10 compared to day 17 across both cell passages.

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Implication

In vitro study reveals altered gene expression and SASP activation in dermal fibroblasts within glycated collagen, highlighting time-dependent matrix effects on cellular senescence.

Cite This Study

Guvatova et al. (2025) studied this question.

synapsesocial.com/papers/69de8e04210a0977fce94ac5 https://doi.org/10.3390/ijms26104769

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Implication

In vitro study reveals altered gene expression and SASP activation in dermal fibroblasts within glycated collagen, highlighting time-dependent matrix effects on cellular senescence.

Changes in Gene Expression Patterns in Young and Senescent Fibroblasts in Glycated Three-Dimensional Collagen Matrices

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Discussion

Implication

Cite This Study

Changes in Gene Expression Patterns in Young and Senescent Fibroblasts in Glycated Three-Dimensional Collagen Matrices

Key Points

Discussion

Implication

Cite This Study

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