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Several important characteristics of the sockeye salmon virus have been determined. It replicates abundantly in cultures of sockeye salmon cells, producing characteristic cytopathic effects. Most of the new virus produced is released into the culture fluid. The particle size was estimated by membrane filtration to be in the range of 110-165 mμ. The maximum rate of virus replication was found in the temperature range from 13 to 18°, and no replication occurred at 23°, although host cells grew well at that temperature. Virus infectivity was destroyed by ether, indicating the presence of essential lipids. Resistance of the agent to inhibition by 5-bromodeoxyuridine offers presumptive evidence that its nucleic acid is RNA.
Wingfield et al. (Tue,) studied this question.