What question did this study set out to answer?

To determine whether human plasma lipoproteins transport Hedgehog morphogens and evaluate their biological function in maintaining endothelial integrity.

September 14, 2010Journal of Proteome Research

Human Plasma Very Low Density Lipoprotein Carries Indian Hedgehog

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Authors

KQKarla QueirozMimetas (Netherlands)RTRené A. TioCardiac Imaging CZClark J. ZeebregtsUniversity Medical Center Groningen

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Implication

Laboratory study reveals that very low density lipoprotein carries Indian Hedgehog in human plasma, suggesting a critical role in preserving endothelial survival and vessel health.

Key Points

To determine whether human plasma lipoproteins transport Hedgehog morphogens and evaluate their biological function in maintaining endothelial integrity.
Analyzed purified human plasma lipoprotein fractions (VLDL, LDL, and HDL) using LTQ-Orbitrap XL mass spectrometry to detect Hedgehog morphogen isoforms.
Investigated Ihh expression in human primary white adipocytes using transcriptome and confocal immunofluorescence analysis.
Assessed cell survival in primary human endothelial cells incubated with VLDL particles.
Indian Hedgehog (Ihh) was uniquely detected in the human VLDL fraction, whereas Sonic and Desert Hedgehog were not detected in any lipoprotein fractions.
Primary white adipocytes demonstrated high Ihh expression at both transcript and protein levels, indicating they serve as the primary source for VLDL-Ihh loading.
VLDL exposure significantly improved primary endothelial cell survival, supporting an active role for circulating Ihh-bearing VLDL in vascular maintenance.

Cite This Study

Queiroz et al. (2010) studied this question.

synapsesocial.com/papers/69decbc9499d77a496b0ce02 https://doi.org/10.1021/pr100403q

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Implication

Laboratory study reveals that very low density lipoprotein carries Indian Hedgehog in human plasma, suggesting a critical role in preserving endothelial survival and vessel health.

Human Plasma Very Low Density Lipoprotein Carries Indian Hedgehog

Discussion

Member takes

Implication

Key Points

Cite This Study

Human Plasma Very Low Density Lipoprotein Carries Indian Hedgehog

Discussion

Member takes

Implication

Key Points

Cite This Study

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