What question did this study set out to answer?

To evaluate the mechanisms governing the transition from HIF-1 to HIF-2 and characterize their target gene expression patterns in primary human endothelial cells during hypoxia.

March 27, 2019The FASEB JournalOpen Access

Primary endothelial cell–specific regulation of hypoxia‐inducible factor (HIF)‐1 and HIF‐2 and their target gene expression profiles during hypoxia

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To evaluate the mechanisms governing the transition from HIF-1 to HIF-2 and characterize their target gene expression patterns in primary human endothelial cells during hypoxia.
Examined the HIF-1 to HIF-2 switch in 10 distinct primary human endothelial cell lines derived from different vascular beds during early-stage hypoxia.
Performed 4 genome-wide mRNA expression arrays in human umbilical vein endothelial cells (HUVECs) under normoxia and after 2, 8, and 16 hours of hypoxia, followed by bioinformatics analyses.
The transition from HIF-1 to HIF-2 represents a universal adaptive mechanism across all 10 endothelial cell types, driven in part by differences in HIF1A and HIF2A mRNA stability.
Bioinformatics analysis demonstrated that while unique gene subsets are regulated exclusively by HIF-1 or HIF-2, the majority of hypoxia-responsive genes are co-regulated by both transcription factors.

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Implication

Laboratory study reveals a universal hypoxia-inducible factor switch from HIF-1 to HIF-2 in human endothelial cells, indicating distinct temporal regulation of hypoxic gene networks.

Cite This Study

Bartoszewski et al. (2019) studied this question.

synapsesocial.com/papers/69deef865e217d93a5559093 https://doi.org/10.1096/fj.201802650rr

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Implication

Laboratory study reveals a universal hypoxia-inducible factor switch from HIF-1 to HIF-2 in human endothelial cells, indicating distinct temporal regulation of hypoxic gene networks.

Primary endothelial cell–specific regulation of hypoxia‐inducible factor (HIF)‐1 and HIF‐2 and their target gene expression profiles during hypoxia

Key Points

Discussion

Implication

Cite This Study

Primary endothelial cell–specific regulation of hypoxia‐inducible factor (HIF)‐1 and HIF‐2 and their target gene expression profiles during hypoxia

Key Points

Discussion

Implication

Cite This Study

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